All products for:ImmunoassayOther
  • VIDAS 25 OH Vitamin D Total

VIDAS® 25 OH Vitamin D Total

Investigation of bone disease

VIDAS® 25 OH Vitamin D Total is an automated test for accurate determination of a patient’s vitamin D status.

  • Used as an aid in the assessment of Vitamin D sufficiency and supplementation follow-up
  • Detection of both vitamin D2 and D3 for reliable results
  • Cost-effective solution
  • Easy to perform in your own lab
Add to my selection

Do you need more information

VIDAS® 25 OH Vitamin D Total is an automated quantitative test for the determination of 25-hydroxyvitamin D Total in human serum or plasma using the ELFA (Enzyme Linked Fluorescent Assay) technique. It reflects vitamin D produced cutaneously and that obtained from food and supplements for a reliable indication of vitamin D status.

An assay of general health status

Vitamin D helps the body absorb and use calcium and phosphorus. As a result, vitamin D deficiency causes bone-weakening diseases such as rickets in children and osteoporosis in adults. Recent studies have also established a link between low circulating vitamin D levels and an increased risk of cancer, diabetes and several cardiovascular or autoimmune diseases.(1)

Beyond bone health, sufficient exposure to vitamin D - through sun exposure, food and dietary supplements - is essential to strengthen the immune system and maintain general health for optimum wellness(2).



The VIDAS® 25 OH Vitamin D Total reference range is compliant with international clinical recommendations(3). It enables clinicians to confidently establish a patient’s vitamin D status and determine the appropriate supplementation when needed. Following the patient’s status will allow to check the dosage and adjust it.


VIDAS® 25 OH Vitamin D Total is very well correlated to the Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) reference method.*

It offers the same high degree of precision and is easy to perform in any lab for rapid results.



LC-MS/MS 25-OH Vitamin D (ng/mL) - Click to enlarge 


For the clinical study 343 samples were tested and the correlation between VIDAS 25 OH Vitamin D Total and LC-MS/MS is Y=1.00x+ 041;R=0.86.

* See package insert for performance details.

Test benefits

Cost-effective solution

  • Single-test format
  • Calibration and control only once a month
  • Kit size adapted to your needs
  • Everything needed to perform the test provided in the kit

Easy to use

  • Automated assay
  • Performed on the instruments of the VIDAS family, well known for their proven reliability
  • Ready-to-use calibrators and controls



1. HOLICK, M.F. (2007). Vitamin D deficiency. N Engl J Med,357:266-281.
2. GRANT WB, HOLICK MF. Benefits and requirements of vitamin D for optimal health: a review. Altern Med Rev. 2005 Jun;10(2):94-111.
3. INSTITUTE OF MEDICINE. Dietary Reference Intakes for Calcium and Vitamin D. Washington, D.C.:
National Academies Press, 2010.

Technical specifications for VIDAS® 25 OH Vitamin D Total
Reference 30463
Tests  / kit 60
Time to result 40 minutes
Sample type Plasma or serum
Sample volume 100 µL 
Calibration frequency 28 days
Cross reaction 25 OH Vitamin D3 100%
25 OH Vitamin D2 91%


Find more technical details on


Consult your local bioMérieux representative for product availability in your country.

Pioneering Diagnostics